Synthesis of Bioreducible Polycations with Controlled Topologies

Ye-Zi You; Jun-Jie Yan; Fei Yu; Zhi-Qiang Yu; David Oupicky

doi:10.1007/978-1-4939-9092-4_2

Synthesis of Bioreducible Polycations with Controlled Topologies

Methods Mol Biol. 2019:1943:27-38. doi: 10.1007/978-1-4939-9092-4_2.

Authors

Ye-Zi You¹, Jun-Jie Yan¹, Fei Yu², Zhi-Qiang Yu¹, David Oupicky³

Affiliations

¹ CAS Key Lab of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, China.
² University of Nebraska Medical Center, Omaha, NE, USA.
³ University of Nebraska Medical Center, Omaha, NE, USA. david.oupicky@unmc.edu.

PMID: 30838607
DOI: 10.1007/978-1-4939-9092-4_2

Abstract

Bioreducible polycations, which possess disulfide linkages in the backbone, have emerged as promising nucleic acid delivery carriers due to their high stability in extracellular physiological condition and bioreduction-triggered release of the genetic material. Further benefits of bioreducible polycations include decreased cytotoxicity due to intracellular reducing environment in the cytoplasm that contains high levels of reducing molecules such as glutathione. Here, we describe the synthesis of bioreducible polycations with emphasis on methods to control their topology.

Keywords: Bioreducible polycations; Controlled topologies; Michael-addition polymerization; Poly(2-(dimethylamino)ethyl methacrylate; Poly(amido amine)s; Polyethylenimine.

MeSH terms

Cytoplasm / chemistry
Drug Carriers / chemical synthesis*
Glutathione / chemistry
Molecular Structure
Nanoparticles / chemistry
Nucleic Acids / genetics
Oxidation-Reduction
Polyamines / chemical synthesis*
Polyelectrolytes
Polyethyleneimine / chemistry
Polymerization*
Temperature

Substances

Drug Carriers
Nucleic Acids
Polyamines
Polyelectrolytes
polycations
Polyethyleneimine
Glutathione