Determination of prostate cancer biomarker acid phosphatase at a copper phthalocyanine-modified screen printed gold transducer

Zina Fredj; Mounir Ben Ali; Mohammed Nooredeen Abbas; Eithne Dempsey

doi:10.1016/j.aca.2018.12.058

Determination of prostate cancer biomarker acid phosphatase at a copper phthalocyanine-modified screen printed gold transducer

Anal Chim Acta. 2019 May 30:1057:98-105. doi: 10.1016/j.aca.2018.12.058. Epub 2019 Jan 9.

Authors

Zina Fredj¹, Mounir Ben Ali¹, Mohammed Nooredeen Abbas², Eithne Dempsey³

Affiliations

¹ University of Sousse, Higher Institute of Applied Sciences and Technology of Sousse, GREENS-ISSAT, 4003, Ibn Khaldoun, Sousse, Tunisia; NANOMISENE Lab, LR 16CRMN01, Centre for Research on Microelectronics and Nanotechnology of Sousse, Technopole of Sousse, Sahloul, 4034, Sousse, Tunisia.
² Electroanal. Lab., National Research Centre (NRC), El Bohouth St., Dokki, 12622 Giza, Egypt.
³ Department of Chemistry, Maynooth University, Maynooth, Co. Kildare, Ireland. Electronic address: eithne.dempsey@mu.ie.

PMID: 30832923
DOI: 10.1016/j.aca.2018.12.058

Abstract

In this work, a novel sensor based on immobilised copper phthalocyanine, 2,9,16,23-tetracarboxylic acid-polyacrylamide (Cu(II)TC Pc-PAA) was developed for determination of acid phosphatase (ACP) levels in nanomolar quantities. Detection was based on the measurement of enzymatically generated phosphate, with initial studies focused on phosphate detection at a Cu(II)TC Pc-PAA modified screen-printed gold transducer. The sensor was characterised in relation to operational performance (pH, response time, stability, linearity, and sensitivity) and common anionic interferents (nitrate, sulphate, chloride, and perchlorate). The functionalised surface also facilitated rapid detection of the enzyme bi-product 2-naphthol over the range 5-3000 μM. Quantitation of ACP was demonstrated, realising a linear response range of 0.5-20 nM and LOD of 0.5 nM, which is within the clinical range for this prostate cancer biomarker.

Keywords: Acid phosphatase; Copper phthalocyanine; Gold screen-printed electrode; Naphthol; Phosphate.

MeSH terms

Acid Phosphatase / analysis*
Biomarkers, Tumor / analysis*
Electrochemistry
Electrodes
Gold / chemistry*
Humans
Hydrogen-Ion Concentration
Indoles / chemistry*
Limit of Detection
Male
Organometallic Compounds / chemistry*
Perchlorates / chemistry
Printing*
Prostatic Neoplasms / metabolism*
Surface Properties
Time Factors
Transducers*

Substances

Biomarkers, Tumor
Indoles
Organometallic Compounds
Perchlorates
copper phthalocyanine
Gold
Acid Phosphatase
Perchloric Acid