Keeping the shape of plant tissue for visualizing metabolite features in segmentation and correlation analysis of imaging mass spectrometry in Asparagus officinalis

Ryo Nakabayashi; Kei Hashimoto; Kiminori Toyooka; Kazuki Saito

doi:10.1007/s11306-019-1486-5

Keeping the shape of plant tissue for visualizing metabolite features in segmentation and correlation analysis of imaging mass spectrometry in Asparagus officinalis

Metabolomics. 2019 Feb 14;15(2):24. doi: 10.1007/s11306-019-1486-5.

Authors

Ryo Nakabayashi¹, Kei Hashimoto², Kiminori Toyooka², Kazuki Saito^{2

3}

Affiliations

¹ RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan. ryo.nakabayashi@riken.jp.
² RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan.
³ Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8675, Japan.

Abstract

Introduction: Matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) is a powerful approach for visualizing the localization of metabolites.

Objectives: A method to keep the shape of plant tissue needs to be developed for MALDI-IMS.

Methods: The method was developed using transfer tape and double-sided conductive tape.

Results: MALDI-IMS analysis using the developed method enabled to perform segmentation and correlation analysis of mass features.

Conclusion: This proof-of-concept study showed that rutin localizes in the epidermis, developing tissue, and protoxylem in Asparagus officinalis.

Keywords: Asparagus officinalis; Imaging mass spectrometry; Liquid chromatography−tandem mass spectrometry; Metabolomics; Specialized metabolite.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Asparagus Plant / metabolism*
Cell Shape / physiology*
Proof of Concept Study
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*