Novel calcium encapsulated mesocellular siliceous foams for crystal growth in dentinal tubules

Yin-Lin Wang; Yu-Chih Chiang; Hao-Hueng Chang; Hong-Ping Lin; Chun-Pin Lin

doi:10.1016/j.jdent.2019.02.007

Novel calcium encapsulated mesocellular siliceous foams for crystal growth in dentinal tubules

J Dent. 2019 Apr:83:61-66. doi: 10.1016/j.jdent.2019.02.007. Epub 2019 Feb 27.

Authors

Yin-Lin Wang¹, Yu-Chih Chiang¹, Hao-Hueng Chang¹, Hong-Ping Lin², Chun-Pin Lin³

Affiliations

¹ School of Dentistry and Graduate Institute of Clinical Dentistry, National Taiwan University and National Taiwan University Hospital, No. 1, Chang-Te Street, Taipei 10016, Taiwan.
² Department of Chemistry, National Cheng Kung University, No. 1, Daxue Rd., Tainan City, 701, Taiwan.
³ School of Dentistry and Graduate Institute of Clinical Dentistry, National Taiwan University and National Taiwan University Hospital, No. 1, Chang-Te Street, Taipei 10016, Taiwan. Electronic address: pinlin@ntu.edu.tw.

PMID: 30825570
DOI: 10.1016/j.jdent.2019.02.007

Abstract

Objectives: The aim of this study was to investigate the novel mesocellular siliceous foams (MCF) containing CaCO₃ nanoparticles (denoted as CMCF) combined with phosphoric acid could occlude dentinal tubules through the formation of biomimetic crystal barrier.

Methods: Ultrastructures of MCF and CMCF were examined by transmission electron microscopy (TEM). Elemental components were analyzed with energy dispersive X-ray spectrometry (EDX). CMCF was mixed with distilled water, 10%, 20% and 30% phosphoric acid then applied on dentine discs. Crystals were characterized by X-ray powder diffractometry (XRD). The sealing efficacy of the dentinal tubules was examined by scanning electron microscopy.

Results: TEM images showed MCF presented a pore size of approximately 30.0 nm and CMCF contained abundant nano-CaCO₃. Sealing efficacy showed that CMCF, when reacted with 30% phosphoric acid, would form crystals in the dentinal tubules to a depth of 83.2 ± 17.6 μm at an occlusion percentage of 75.6 ± 12.8% on average; both occlusion percentage and depth were higher than those obtained with 10% or 20% phosphoric acid (p < 0.05). The results of XRD and EDS indicated that the crystal growth in the dentinal tubules could be transformed into the biomimetic crystals.

Conclusion: This study showed that the CMCF with 30% phosphoric acid could effectively occlude the dentinal tubules through the formation of biomimetic crystal barrier.

Clinical significance: The novel CMCF combined with phosphoric acid may have potential for the treatment of dentine hypersensitivity.

Keywords: Calcium phosphates; Crystal growth; Dentinal tubule; Dicalcium phosphate dihydrate; Mesocellular siliceous foams.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Calcium*
Crystallization
Dentin / chemistry*
Dentin Sensitivity*
Humans
Microscopy, Electron, Scanning

Substances

Calcium