Steady-State and Pulse-Chase Analyses of Fibrillar Collagen

Antonella Forlino; Francesca Tonelli; Roberta Besio

doi:10.1007/978-1-4939-9133-4_4

Steady-State and Pulse-Chase Analyses of Fibrillar Collagen

Methods Mol Biol. 2019:1952:45-53. doi: 10.1007/978-1-4939-9133-4_4.

Authors

Antonella Forlino¹, Francesca Tonelli², Roberta Besio²

Affiliations

¹ Biochemistry Unit, Department of Molecular Medicine, University of Pavia, Pavia, Italy. aforlino@unipv.it.
² Biochemistry Unit, Department of Molecular Medicine, University of Pavia, Pavia, Italy.

PMID: 30825164
DOI: 10.1007/978-1-4939-9133-4_4

Abstract

Steady-state and pulse-chase collagen analyses are powerful approaches to investigate in vitro the structure of collagen and its kinetic of secretion, respectively. The electrophoretic analysis of purified ³H-proline-labeled collagen allows to determine the nature and post translational modifications of its α chains, whereas short-pulse labeling can be used to follow collagen secretion over time.

Keywords: Collagen; Osteogenesis imperfecta; Post translational modifications; Pulse-chase; Steady-state.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Electrophoresis, Polyacrylamide Gel / methods*
Fibrillar Collagens / chemistry*
Fibroblasts / chemistry
Humans
Proline / analysis
Protein Processing, Post-Translational
Tritium / analysis
Urea / chemistry

Substances

Fibrillar Collagens
Tritium
Urea
Proline