Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection

Methods Mol Biol. 2019:1951:135-141. doi: 10.1007/978-1-4939-9130-3_10.

Abstract

Macrophages are phagocytic cells that actively engulf and kill microorganisms within a specialized phagolysosomal system. Several pathogenic bacteria, however, actively co-opt host mechanisms and escape from microbial digestion to establish intracellular replication within macrophages. This chapter highlights detailed protocols to measure the effects of the LXR pathway on bacterial infection of murine bone marrow-derived macrophages.

Keywords: Bacteria; Infection; LXR; Macrophage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Infections / metabolism*
  • Bacterial Infections / microbiology
  • Biomarkers
  • Flow Cytometry
  • Immunophenotyping
  • Liver X Receptors / agonists*
  • Liver X Receptors / metabolism*
  • Macrophages / drug effects*
  • Macrophages / metabolism*
  • Mice
  • Microscopy, Confocal
  • Orphan Nuclear Receptors / agonists
  • Orphan Nuclear Receptors / metabolism

Substances

  • Biomarkers
  • Liver X Receptors
  • Orphan Nuclear Receptors