Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A

Mingyue Piao; Donglei Zou; Yuesuo Yang; Xianghao Ren; Chuanyu Qin; Yunxian Piao

doi:10.3390/ma12050704

Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A

Materials (Basel). 2019 Feb 27;12(5):704. doi: 10.3390/ma12050704.

Authors

Mingyue Piao^{1

2}, Donglei Zou³, Yuesuo Yang^{4

5}, Xianghao Ren⁶, Chuanyu Qin⁷, Yunxian Piao⁸

Affiliations

¹ Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun 130021, China. piaomingyue1985@163.com.
² College of Environmental Science and Engineering, Jilin Normoal University, 1301 Haifeng Road, Siping 136000, China. piaomingyue1985@163.com.
³ Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun 130021, China. zoudl@jlu.edu.cn.
⁴ Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun 130021, China. yangyuesuo@jlu.edu.cn.
⁵ Key Laboratory of Eco-restoration of Regional Contaminated Environment, Ministry of Education, Shenyang University, Shenyang 110044, China. yangyuesuo@jlu.edu.cn.
⁶ Key Laboratory of Urban Storm water System and Water Environment, Ministry of Education, School of Environment and Energy Engineering, Beijing University of Civil Engineering and Architecture, Beijing 100044, China. Renxianghao@bucea.edu.cn.
⁷ Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun 130021, China. qincyu@jlu.edu.cn.
⁸ Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun 130021, China. yxpiao@jlu.edu.cn.

Abstract

Hghly stable, reusable, and multi-functional biocatalytic microparticles with Laccase (Lac) enzyme (Lac/particles) were synthesized for bisphenol A (BPA) removal from aqueous solution. The Lac/particles were prepared by encapsulating Lac enzymes into poly ethylene glycol (PEG) hydrogel via the UV assisted emulsion polymerization method followed by cross linking with glutaraldehyde (GA). The obtained Lac/particles were spherical and micron sized (137⁻535 μm), presenting high enzyme entrapment efficiency of 100%, high activity recovery of 18.9%, and great stability at various pHs (3⁻7) than the free Lac. The Lac/particles could adsorb the BPA into the catalytic particles in a short time, promoting contact between BPA and enzyme, and further enzymatically degrade them without the shaking process and independent surrounding buffer solution. The Lac/particles could be reused for another round BPA adsorption and biotranformation by maintaining over 90% of BPA removal efficiency after seven times reuse. The synergistic effects of adsorption and biocatalytical reaction of Lac/particles have significant values in high efficient and cost-effective BPA removal.

Keywords: bisphenol a removal; entrapment; hydrogel microparticle; laccase.

Abstract

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