In vivo detecting mouse persistent hyperplastic primary vitreous by Spectralis Optical Coherence Tomography

Qing Lian; Minglei Zhao; Tianzhong Li; Keling Wu; Deliang Zhu; Bizhi Shang; Tingfang Mei; Weihua Li; Ying Lin; Fuxiang Mao; Yizhi Liu; Chujun Liu; Lin Lu; Ling Zhao

doi:10.1016/j.exer.2019.02.017

In vivo detecting mouse persistent hyperplastic primary vitreous by Spectralis Optical Coherence Tomography

Exp Eye Res. 2019 Apr:181:271-276. doi: 10.1016/j.exer.2019.02.017. Epub 2019 Feb 25.

Authors

Qing Lian¹, Minglei Zhao¹, Tianzhong Li¹, Keling Wu¹, Deliang Zhu¹, Bizhi Shang¹, Tingfang Mei¹, Weihua Li¹, Ying Lin¹, Fuxiang Mao¹, Yizhi Liu¹, Chujun Liu², Lin Lu³, Ling Zhao⁴

Affiliations

¹ State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
² State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China. Electronic address: liuchujun@hotmail.com.
³ State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China. Electronic address: drlulin@126.com.
⁴ State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China; Guangdong Province Key Laboratory of Brain Function and Disease, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China. Electronic address: zhaoling6@mail.sysu.edu.cn.

PMID: 30817926
DOI: 10.1016/j.exer.2019.02.017

Abstract

To identify imaging characteristics of mouse persistent hyperplastic primary vitreous (PHPV) by Spectralis Optical Coherence Tomography (OCT), as well as to assess and compare the sensitivity and precision of OCT with color photography (CP) and Fundus Fluorescein Angiography (FFA) imaging in detecting mouse PHPV. Notch4^-/- C57BL/6J mice (224 eyes) aged from 3 months to 7 months were examined in this study. CP, FFA and OCT imaging were utilized to examine vitreous cavity and retina of mouse eyes. Horizontal and radial OCT scan volume was centered on the optic nerve head. Hematoxylin and eosin (H&E) staining was performed to validate PHPV. For color photography and FFA imaging, retrolental irregular fibrovascular membrane-like tissues were found in 33 eyes with/without blood vessels in vitreous cavity. Among them, 31 eyes were visualized with lateral and oblique linear hyperreflective opacities in vitreous cavity using Spectralis OCT. Position of PHPV in posterior segment of eyes was also measured via OCT. Mouse PHPV was validated by H&E staining. Typical hyperreflective opacities in vitreous cavity were detected in PHPV mouse using Spectralis OCT. Spectralis OCT imaging can effectively detect mouse PHPV as color photography and FFA.

Keywords: Hyperreflective opacities; Mouse; Optical coherence tomography; Persistent hyperplastic primary vitreous; Vitreous disease.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Disease Models, Animal
Disease Progression
Feasibility Studies
Fluorescein Angiography / methods
Fundus Oculi
Mice
Mice, Inbred C57BL
Optic Disk / pathology
Persistent Hyperplastic Primary Vitreous / diagnosis*
Severity of Illness Index
Tomography, Optical Coherence / methods*
Vitreous Body / pathology*