Susceptibility of Brazilian Populations of Chrysodeixis includens (Lepidoptera: Noctuidae) to Selected Insecticides

Abstract

Soybean looper (SBL), Chrysodeixis includens (Walker) (Lepidoptera: Noctuidae), is an important pest of soybean and cotton in Brazil. The use of insecticides is one of the main control tactics against this pest. To support Integrated Pest Management (IPM) and Insect Resistance Management (IRM) programs, we characterized the susceptibility of Brazilian populations of SBL to insecticides. Field populations were collected from soybean fields during the 2016-2017 and 2017-2018 crop seasons. In the laboratory, late L2/early L3 larvae were exposed to insecticides in diet-overlay or topical bioassays. Field populations of SBL showed high susceptibility to spinetoram (LC50 = 0.074-0.25 μg a.i. per cm2), indoxacarb (LC50 = 0.46-0.94 μg a.i. per cm2), thiodicarb (LC50 = 9.14-36.61 μg a.i. per cm2), chlorantraniliprole (LC50 = 0.15-0.57 μg a.i. per cm2), flubendiamide (LC50 = 0.45-2.01 μg a.i. per cm2), and chlorfenapyr (LC50 = 0.15-0.25 μg a.i. per cm2); the resistance ratios were less than 16-fold. In contrast, SBL populations have reduced susceptibility to lambda-cyhalothrin (LC50 = 3.71-9.54 μg a.i./cm2), methoxyfenozide (LC50 = 0.67-4.23 μg a.i. per cm2), novaluron (LC50 = 27.52-77.63 μg a.i. per cm2), and teflubenzuron (LC50 = 13.41-73.02 μg a.i. per cm2). The resistance ratios relative to a Lab population (susceptible of reference) was up to 38-, 63-, 1,553-, and 5,215-fold, respectively. These high resistance ratios can be associated with resistance evolution. Implications of these findings to IPM and IRM programs are discussed.

Keywords: chemical control; resistance management; soybean looper; toxicity.