A new, simple, rapid, highly sensitive and selective and non-enzymatic fluorometric method for direct determination of glucose in real samples was developed. The method was based on the inhibition of fluorescence resonance energy transfer (FRET) process between terbium (III)-1, 10-phenanthroline (Tb-phen) complex and silver nanoparticles (AgNPs). Upon the addition of glucose, the quenched FRET-based fluorescence of Tb-phen complex was gradually recovered by glucose via its strong adsorption on the surface of AgNPs and removal of Tb-phen complex from AgNPs surface. Therefore the fluorescence of Tb-phen complex switched to "turn-on" state. Under the optimum conditions, a linear relationship was obtained between the enhanced fluorescence intensity and glucose concentration in the range of (5-900) × 10-8 M with the detection limit of 1.94 × 10-8 M. The proposed sensing system was successfully applied to determine glucose in the spiked normal and diabetic patient serum samples after deproteinization with acetonitrile. Analytical recoveries from treated serum samples were in the range of 99.97-104.80% and 92.14-105.43%, respectively. The common interfering species, such as ascorbic acid, fructose and galactose did not cause interior interference due to unique emission properties of Tb-phen complex probe. Also the interaction of the Tb-phen complex with AgNPs, which led to the fluorescence intensity quenching of the complex, was further examined by FTIR technique. In short, as compared to most of the existing methods, the newly proposed method, provides some advantages and makes it promising for the direct rapid screening of glucose residues of real samples in clinical diagnosis of diabetes, as an alternative approach to the other exiting optical and electrochemical methods.
Keywords: Diabetes mellitus; FRET-based nanosensor; Glucose; Tb-phen complex; Turn on/off.
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