The cholera continues to be one of the priority problems of world health care that determines necessity of its permanent monitoring and also further investigation of this infection in microbiological and ecological aspects. The phenomenon of biofilm formation plays a significant role among mechanisms permitting comma bacillus year by year to retain its ecological niche and to spread in various basins over a period of decades. The application of techniques of electronic microscopy played a decisive role in studying the process of biofilm formation. The transmission electronic microscopy has a number of significant advantages, but studying of biofilms using the given technique has certain limitations and peculiarities affecting derivable results. The purpose of study is modeling of biofilms of comma bacillus using a new original technique and analyzing them implementing transmission electronic microscopy. An original substrate was developed to cultivate biofilms of comma bacillus adjusted to convenient sampling preparation for transmission electronic microscopy. The application of the given technique manifold simplifies the process sampling preparation and permits to retain at most the native structure of biofilms. By means of combining alternatives of contrasting and comparing of obtained results the role of various components in the process of biofilm formation is determined. Also, the structure of matrix of biofilms and morphologic phasic picture of process of biofilm formation of comma bacillus is demonstrated. The electronic microscopic indications of biofilm forms of comma bacillus are marked out. The presented data supports optimization of studying biofilms using transmission electronic microscopy and better understanding phenomenon of biofilm formation.
Keywords: biofilm; biofilm formation; cultivation of biofilms; transmission electronic microscopy; vibrio cholerae.