CagL polymorphisms D58/K59 are predominant in Helicobacter pylori strains isolated from Mexican patients with chronic gastritis

Adolfo Román-Román; Verónica I Martínez-Santos; Carlos A Castañón-Sánchez; Alan J Albañil-Muñoz; Paola González-Mendoza; Diana G Soto-Flores; Dinorah N Martínez-Carrillo; Gloria Fernández-Tilapa

doi:10.1186/s13099-019-0286-9

CagL polymorphisms D58/K59 are predominant in Helicobacter pylori strains isolated from Mexican patients with chronic gastritis

Gut Pathog. 2019 Feb 13:11:5. doi: 10.1186/s13099-019-0286-9. eCollection 2019.

Authors

Adolfo Román-Román^#¹, Verónica I Martínez-Santos^#², Carlos A Castañón-Sánchez³, Alan J Albañil-Muñoz⁴, Paola González-Mendoza⁴, Diana G Soto-Flores⁴, Dinorah N Martínez-Carrillo⁴, Gloria Fernández-Tilapa⁴

Affiliations

¹ 1Laboratorio de Investigación en Bacteriología, Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas s/n C.U. Sur., C.P. 39090 Chilpancingo, Guerrero Mexico.
² 2Universidad Autónoma de Guerrero, Av. Javier Méndez Aponte No. 1, Fracc. 10, Col. Servidor Agrario, C.P. 39070 Chilpancingo, Guerrero Mexico.
³ Hospital Regional de Alta Especialidad de Oaxaca, Aldama s/n, Col. Centro, C.P. 71256 San Bartolo Coyotepec, Oaxaca Mexico.
⁴ 4Laboratorio de Investigación Clínica, Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas s/n C.U. Sur., C.P. 39090 Chilpancingo, Guerrero Mexico.

^# Contributed equally.

Abstract

Background: Helicobacter pylori is a Gram-negative bacterium that colonizes the gastric mucosa in humans. One of the main virulence factors of H. pylori is the cag pathogenicity island (cagPAI), which encodes a type 4-secretion system (T4SS) and the cytotoxin CagA. Translocation of CagA through the T4SS triggers host-signaling pathways. One of the T4SS proteins is CagL, which is necessary for CagA translocation. CagL is a 26-kDa protein that contains a hypervariable motif, which spans residues 58 to 62. Several polymorphisms in this region have been associated with different disease outcomes, e.g. in Mexico, N58 is associated with a higher risk of gastric cancer. The aim of this work is to analyze the sequence of the hypervariable motif (residues 58 to 62) of clinical isolates from Mexican patients with chronic gastritis, and to correlate these polymorphisms with the vacA genotype.

Results: Of the 164 biopsies analyzed, only 30.5% (50/164) were positive for H. pylori. Thirty-six of the 50 clinical isolates (72%) were cagA positive, and 40 (80%) had the most virulent vacA genotype (s1/m1). Of the cagA positive strains, 94.4% were vacA s1/m1. All the cagA ⁺ strains contained the cagL gene. The most prevalent sequence in the polymorphic region (residues 58-62) was DKMGE (75.8%, 25/33), followed by NKMGQ and NEIGQ (6.1%, 2/33), and DEIGQ, NKMGE, DKIGE, and DKIGK (3%, 1/33). Regarding polymorphisms in positions 58 and 59, the most common were D58/K59 (81.8%, 27/33), followed by N58/K59 (9.1%, 3/33), and D58/E59 (3%, 1/33). Only two isolates (6.1%) contained residues N58/E59, which correspond to those found in H. pylori strain ATCC 26695. 92.6% of the clinical isolates having polymorphism D58/K59 had the genotype vacA s1/m1, considered to be the most virulent, while 7.4% had the genotypes vacA s1/m2 and s2/m2.

Conclusions: In Mexican patients, CagL polymorphisms D58, K59, M60, E62, K122, and I134 are more common in patients with chronic gastritis.

Keywords: Chronic gastritis; H. pylori; Polymorphic region; cagL polymorphisms.