Logic Regression Analysis of Gene Polymorphisms and HDL Levels in a Nationally Representative Sample of Iranian Adolescents: The CASPIAN-III Study

Mehri Moghadasi; Roya Kelishadi; Hamid Reza Marateb; Shaghayegh Haghjooy Javanmard; Marjan Mansourian; Ramin Heshmat; Mohammad Esmaeil Motlagh

doi:10.5812/ijem.14037

Logic Regression Analysis of Gene Polymorphisms and HDL Levels in a Nationally Representative Sample of Iranian Adolescents: The CASPIAN-III Study

Int J Endocrinol Metab. 2017 Jul 29;15(3):e14037. doi: 10.5812/ijem.14037. eCollection 2017 Jul.

Authors

Mehri Moghadasi¹, Roya Kelishadi², Hamid Reza Marateb^{3

4}, Shaghayegh Haghjooy Javanmard⁵, Marjan Mansourian^{2

5

6}, Ramin Heshmat⁷, Mohammad Esmaeil Motlagh⁸

Affiliations

¹ Student Research Center, Biostatistics and Epidemiology Department, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran.
² Pediatrics Department, Child Growth and Development Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
³ Biomedical Engineering Department, Faculty of Engineering, University of Isfahan, Isfahan, Iran.
⁴ Department of Automatic Control, Biomedical Engineering Research Center, Universitat Politècnica de Catalunya, BarcelonaTech (UPC), Barcelona, Spain.
⁵ Physiology Department, Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
⁶ Biostatistics and Epidemiology Department, Faculty of Health, Isfahan University of Medical Sciences, Isfahan, Iran.
⁷ Chronic Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
⁸ Pediatrics Department, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Abstract

Background: To investigate the associations of genetic polymorphism with high-density lipoprotein-cholesterol (HDL-C) levels in Iranian adolescents.

Methods: This multicentre study was conducted on 10 - 18 year-old students from 27 provinces in Iran. Logic regression approach was used to determine the main effects and interactions of polymorphisms related to HDL-C levels.

Results: The rs708272 polymorphism was significantly related to HDL-C levels. Moreover, rs708272 increased HDL-C levels and had a protective effect on HDL-C. The interaction of rs2230808 and rs5880 polymorphisms as well as the interaction of rs320 and rs708272 polymorphisms were associated with lower HDL-C levels. Furthermore, the interaction of rs320 and rs1801177 polymorphisms was associated with lower HDL-C levels.

Conclusions: We found that not only single SNPs, but also interactions of several SNPs affect HDL-C levels. Given the high prevalence of low HDL-C in Middle Eastern populations, further genetic studies are required for detailed analysis.

Keywords: High-Density Lipoprotein; Logic Regression; Pediatrics; Polymorphism.