Differential response of Indian mustard (Brassica juncea L., Czern and Coss) under salinity: photosynthetic traits and gene expression

Jogendra Singh; Vijayata Singh; T V Vineeth; Parveen Kumar; Neeraj Kumar; Parbodh C Sharma

doi:10.1007/s12298-018-0631-3

Differential response of Indian mustard (Brassica juncea L., Czern and Coss) under salinity: photosynthetic traits and gene expression

Physiol Mol Biol Plants. 2019 Jan;25(1):71-83. doi: 10.1007/s12298-018-0631-3. Epub 2018 Dec 12.

Authors

Jogendra Singh¹, Vijayata Singh¹, T V Vineeth¹, Parveen Kumar¹, Neeraj Kumar¹, Parbodh C Sharma¹

Affiliation

¹ ICAR-Central Soil Salinity Research Institute, Karnal, Haryana 132001 India.

Abstract

To explore the effect of salt stress on photosynthetic traits and gene expression in Indian mustard, four genotypes CS 54 (national check for salinity), CS 52-SPS-1-2012 (salt tolerant mutant), CS 614-4-1-4-100-13 (salt sensitive mutant) and Pusa bold (high yielding variety) were evaluated under irrigation water salinity (EC_iw 12, and 15 dS m^-1). Results suggest genotype CS 52-SPS-1-2012 followed by CS 54 performed better under imposed salt stress due to differential regulation of Na⁺ accumulation in the roots and main stem, restriction of Na⁺ influx from root to shoot, maintaining higher net photosynthetic traits under saline stress compared to CS 614-4-1-4-100-13 and Pusa bold. Further, overexpression of antiporters (SOS1, SOS2, SOS3, ENH1 and NHX1) and antioxidant (APX1, APX4, DHAR1 and MDHAR) genes in salt tolerant genotypes CS 52-SPS-1-2012 and CS 54 demonstrated their significant role in imparting salt tolerance in Indian mustard.

Keywords: Gene expression; Photosynthetic traits; Salinity; Salt tolerant.