Therapeutic effects and anti-metastasis effects of cationic liposomes against pancreatic cancer metastasis in vitro and in vivo

Biochem Biophys Res Commun. 2019 Apr 9;511(3):504-509. doi: 10.1016/j.bbrc.2019.02.116. Epub 2019 Feb 23.

Abstract

The anti-metastatic effects of cationic liposomes (CL) composed of 87 mol% dimyristoylphosphatidylcholine (DMPC), 8 mol% O,O'-ditetradecanoyl-N-(α-trimethylammonioacetyl) diethanolamine chloride (2C14ECl) and 5 mol% polyoxyethylene(21) dodecyl ether (C12(EO)21) was investigated for human pancreatic cancer (BxPC-3) cells. The inhibitory effect of CL on the migration of BxPC-3 cells was observed based on a wound scratch assay. CL suppressed pseudopodium formation of BxPC-3 cells. The anti-invasive effect of CL against BxPC-3 cells was observed via a Matrigel invasion assay. The anti-invasive effect of CL for BxPC-3 cells was found to occur through the inhibition of MMP2, MMP9, and MMP14. Overall, the results of this study revealed for the first time, the therapeutic effects and anti-metastasis activity of CL in xenograft mouse models for peritoneal metastasis of human pancreatic cancer.

Keywords: Anti-metastasis effects; Cationic liposome; In vivo; Pancreatic cancer; Therapeutic effects.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cations / therapeutic use
  • Cell Line, Tumor
  • Cell Movement / drug effects
  • Dimyristoylphosphatidylcholine / therapeutic use*
  • Ethanolamines / therapeutic use*
  • Ethylamines / therapeutic use*
  • Humans
  • Liposomes / therapeutic use*
  • Mice, Inbred BALB C
  • Neoplasm Invasiveness / pathology
  • Neoplasm Invasiveness / prevention & control*
  • Pancreatic Neoplasms / drug therapy*
  • Pancreatic Neoplasms / pathology

Substances

  • Cations
  • Ethanolamines
  • Ethylamines
  • Liposomes
  • O,O'-ditetradecanoyl-N-(alpha-trimethylammonioacetyl) diethanolamine chloride
  • Dimyristoylphosphatidylcholine