Measuring Intracellular Metal Concentration via ICP-MS Following Copper Exposure

Caitlin L Williams; Heather M Neu; Sarah L J Michel; D Scott Merrell

doi:10.1007/978-1-4939-9118-1_19

Measuring Intracellular Metal Concentration via ICP-MS Following Copper Exposure

Methods Mol Biol. 2019:1946:195-205. doi: 10.1007/978-1-4939-9118-1_19.

Authors

Caitlin L Williams¹, Heather M Neu², Sarah L J Michel², D Scott Merrell³

Affiliations

¹ Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
² Department of Pharmaceutical Sciences, University of Maryland, Baltimore, MD, USA.
³ Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA. douglas.merrell@usuhs.edu.

PMID: 30798557
DOI: 10.1007/978-1-4939-9118-1_19

Abstract

This chapter describes protocols for measuring fluctuation in intracellular metal concentration of A. baumannii isolates in response to copper exposure using inductively coupled plasma mass spectrometry (ICP-MS).

Keywords: A. baumannii; Acinetobacter; Copper; Heavy metal; ICP-MS; Intracellular; Mass spectrometry.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Acinetobacter baumannii / drug effects
Acinetobacter baumannii / metabolism
Copper / analysis
Copper / metabolism
Copper / pharmacology
Intracellular Space / metabolism*
Mass Spectrometry* / methods
Metals / analysis*
Metals / metabolism*

Substances

Metals
Copper