Testing Metal Sensitivity of A. baumannii Strains: Survival in Copper-Supplemented Liquid Media and on Copper-Containing Surfaces

Caitlin L Williams; D Scott Merrell

doi:10.1007/978-1-4939-9118-1_5

Testing Metal Sensitivity of A. baumannii Strains: Survival in Copper-Supplemented Liquid Media and on Copper-Containing Surfaces

Methods Mol Biol. 2019:1946:51-60. doi: 10.1007/978-1-4939-9118-1_5.

Authors

Caitlin L Williams¹, D Scott Merrell²

Affiliations

¹ Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
² Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA. douglas.merrell@usuhs.edu.

PMID: 30798543
DOI: 10.1007/978-1-4939-9118-1_5

Abstract

This chapter describes protocols for determining A. baumannii isolates' overall levels of sensitivity to heavy metals; copper is used as a model heavy metal. Measurements of the ability of strains to grow in the presence of various concentrations of copper in liquid media and on copper-containing surfaces are described.

Keywords: A. baumannii; Acinetobacter; Copper; Coupon; Heavy metal; Surface.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Acinetobacter baumannii / drug effects*
Acinetobacter baumannii / growth & development
Acinetobacter baumannii / metabolism
Copper / metabolism
Copper / pharmacology
Culture Media
Dose-Response Relationship, Drug
Humans
Metals, Heavy / metabolism
Metals, Heavy / pharmacology*
Microbial Sensitivity Tests*
Microbial Viability / drug effects

Substances

Culture Media
Metals, Heavy
Copper