Nybomycin-producing Streptomyces isolated from carpenter ant Camponotus vagus

Yuliya V Zakalyukina; Mikhail V Birykov; Dmitrii A Lukianov; Dmitrii I Shiriaev; Ekaterina S Komarova; Dmitry A Skvortsov; Yury Kostyukevich; Vadim N Tashlitsky; Vladimir I Polshakov; Eugene Nikolaev; Petr V Sergiev; Ilya A Osterman

doi:10.1016/j.biochi.2019.02.010

Nybomycin-producing Streptomyces isolated from carpenter ant Camponotus vagus

Biochimie. 2019 May:160:93-99. doi: 10.1016/j.biochi.2019.02.010. Epub 2019 Feb 21.

Affiliations

¹ Department of Soil Science, Lomonosov Moscow State University, Moscow, 119234 Russia.
² Department of Biology, Lomonosov Moscow State University, Moscow, 119234 Russia.
³ Skolkovo Institute of Science and Technology, Skolkovo, Moscow Region, 143025, Russia.
⁴ Department of Chemistry and A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119992, Russia.
⁵ Skolkovo Institute of Science and Technology, Skolkovo, Moscow Region, 143025, Russia; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, 119234 Russia.
⁶ Center for Magnetic Tomography and Spectroscopy, Faculty of Fundamental Medicine, M.V., Russia.
⁷ Skolkovo Institute of Science and Technology, Skolkovo, Moscow Region, 143025, Russia; Department of Chemistry and A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119992, Russia.
⁸ Skolkovo Institute of Science and Technology, Skolkovo, Moscow Region, 143025, Russia; Department of Chemistry and A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119992, Russia. Electronic address: i.osterman@skoltech.ru.

PMID: 30797881
DOI: 10.1016/j.biochi.2019.02.010

Abstract

A novel strain of Actinomycetes was isolated from the body of an ant (Camponotus vagus Scopoli) and its genetic and morphological properties were characterized. The 16S rDNA gene sequence analysis of the isolate revealed its high phylogenetic relationship with type strains of Streptomyces violaceochromogenes NBRC 13100^T. As a result of antimicrobial activity assessment, it was found that the fermentation broth of the isolated strain both inhibited the growth and induced the SOS response in E. coli BW25113 ΔtolC strain cells. Using bioassay-guided fractionation, mass spectrometric and NMR analyses we identified the active compound to be nybomycin, a previously described antibiotic. Here we report for the first time Streptomyces producer of nybomycin in association with carpenter ants and demonstrate cytotoxic activity of nybomycin against human cell lines.

Keywords: Actinobacteria; Antibacterial activity; Ants; Camponotus; Nybomycin; Reporter strain; Streptomyces.

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology*
Ants
Cell Survival
Cells, Cultured
DNA, Bacterial / genetics
Escherichia coli / drug effects
Escherichia coli / growth & development*
Fibroblasts / cytology*
Fibroblasts / drug effects
Humans
Lung Neoplasms / drug therapy
Lung Neoplasms / pathology*
Phylogeny
Quinolones / pharmacology
RNA, Ribosomal, 16S / genetics
Streptomyces / isolation & purification
Streptomyces / metabolism*

Substances

Anti-Bacterial Agents
DNA, Bacterial
Quinolones
RNA, Ribosomal, 16S
nybomycin