Synaptobrevin-2 C-Terminal Flexible Region Regulates the Discharge of Catecholamine Molecules

Abstract

The discharge of neurotransmitters from vesicles is a regulated process. Synaptobrevin-2, a snap receptor (SNARE) protein, participates in this process by interacting with other SNARE and associated proteins. Synaptobrevin-2 transmembrane domain is embedded into the vesicle lipid bilayer except for its last three residues. These residues are hydrophilic and constitute synaptobrevin-2 C-terminal flexible region. The residue Y113 of synaptobrevin-2 flexible region was mutated to lysine and glutamate. The effects of these mutations on the exocytotic process in chromaffin cells were assessed using capacitance measurements combined with amperometry and stimulation by flash photolysis of caged Ca²⁺. Both Y113E and Y113K mutations reduced the number of fusion-competent vesicles and reduced the rates of release of catecholamine molecules in quanta release events. These results exclude any direct interaction of this domain with the catecholamine molecules that are escaping through the fusion pore but favor its interaction with the vesicle membrane as a mean of regulating exocytosis.