Unique cartilage matrix-associated protein regulates fibrillin-2 expression and directly interacts with fibrillin-2 protein independent of calcium binding

Yeon-Ju Lee; Seung-Yoon Park; Eui Kyun Park; Jung-Eun Kim

doi:10.1016/j.bbrc.2019.01.128

Unique cartilage matrix-associated protein regulates fibrillin-2 expression and directly interacts with fibrillin-2 protein independent of calcium binding

Biochem Biophys Res Commun. 2019 Apr 2;511(2):221-227. doi: 10.1016/j.bbrc.2019.01.128. Epub 2019 Feb 18.

Authors

Yeon-Ju Lee¹, Seung-Yoon Park², Eui Kyun Park³, Jung-Eun Kim⁴

Affiliations

¹ Department of Molecular Medicine, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu, South Korea; BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, Kyungpook National University, Daegu, South Korea.
² Department of Biochemistry, School of Medicine, Dongguk University, Gyeongju, South Korea.
³ Department of Oral Pathology and Regenerative Medicine, School of Dentistry, IHBR, Kyungpook National University, Daegu, South Korea.
⁴ Department of Molecular Medicine, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu, South Korea; BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, Kyungpook National University, Daegu, South Korea. Electronic address: kjeun@knu.ac.kr.

PMID: 30791984
DOI: 10.1016/j.bbrc.2019.01.128

Abstract

Unique cartilage matrix-associated protein (UCMA) is a secretory γ-carboxyglutamate (Gla) containing protein that is mainly expressed in the cartilage. Ucma, a downstream gene of both Runx2 and Osterix, has recently been described to promote osteoblast differentiation and matrix mineralization. However, till date, no studies have focused on the role of downstream target genes of Ucma in osteogenesis. Here, by Affymetrix GeneChip microarray analysis, we determined 45 differentially expressed genes in response to Ucma stable overexpression or knockdown in osteoblast cells, which provided insight into molecular mechanisms underlying osteoblast differentiation. In particular, we showed that fibrillin-2 (FBN2) expression was proportional to Ucma expression in osteoblasts as validated by quantitative PCR. We also showed that even though Gla-containing UCMA and calcium-binding EGF-like domain-containing FBN2 are known to have a high affinity for calcium, FBN2 whose expression was regulated by UCMA directly interacted with the UCMA protein, independent of calcium.

Keywords: Calcium; Fibrillin-2; Gene profile; Interaction; Microarray; Ucma.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Calcium / metabolism*
Cell Line
Extracellular Matrix Proteins / genetics
Extracellular Matrix Proteins / metabolism*
Fibrillin-2 / genetics
Fibrillin-2 / metabolism*
Gene Expression Regulation
Intracellular Signaling Peptides and Proteins
Mice
Osteoblasts / cytology
Osteoblasts / metabolism*
Osteogenesis
Protein Interaction Maps

Substances

Extracellular Matrix Proteins
Fibrillin-2
Intracellular Signaling Peptides and Proteins
Ucma protein, mouse
Calcium