A highly sensitive fluorescent probe with different responses to Cu2+ and Zn2

Abstract

We report a highly sensitive fluorescent probe based on p-dimethylaminobenzoyl derivatives (probe L) for the detection of Cu²⁺ and Zn²⁺. In this work, the probe L exhibited a fluorescent turn-on sensing model to Cu²⁺ and Zn²⁺ with a distinct fluorescent color change from colorless to green and yellow respectively. Probe L exhibited high selectivity as a fluorescent Cu²⁺/Zn²⁺ probe with a limit of detection (LOD) of 45 nM/17 nM. The results of ¹H NMR titrations revealed that the response of L to Cu²⁺ and Zn²⁺ was triggered by the interaction of the thiophene unit and the metal ion. Furthermore, the fluorescence titrations and Job's plot curves displayed the binding ratio of 1:2 for Cu²⁺ and 1:1 for Zn²⁺ metal-L complex formation respectively. Density functional theory calculation also demonstrated the possibility of molecular luminescence and the process of metal-L complex formation. Additionally, fluorescent test strips have been prepared for convenient detection of Cu²⁺ and Zn²⁺, which means the convenient and rapid assay in real samples can be achieved.

Keywords: Cu(2+); Fluorescence; Multi-target detection; Reversibility; Test strips; Zn(2+).