The minimum information required for a glycomics experiment (MIRAGE) project: LC guidelines

Matthew P Campbell; Jodie L Abrahams; Erdmann Rapp; Weston B Struwe; Catherine E Costello; Milos Novotny; Rene Ranzinger; William S York; Daniel Kolarich; Pauline M Rudd; Carsten Kettner

doi:10.1093/glycob/cwz009

The minimum information required for a glycomics experiment (MIRAGE) project: LC guidelines

Glycobiology. 2019 May 1;29(5):349-354. doi: 10.1093/glycob/cwz009.

Authors

Affiliations

¹ Institute for Glycomics, Griffith University, Gold Coast, Queensland, Australia.
² Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstraße 1, Magdeburg, Germany.
³ Department of Biochemistry, Oxford Glycobiology Institute, University of Oxford, Oxford, UK.
⁴ Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University School of Medicine, 670 Albany Street, Suite 504, Boston, MA, USA.
⁵ Department of Chemistry, Indiana University, 800 E. Kirkwood Avenue, Bloomington, IN, USA.
⁶ Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA, USA.
⁷ ARC Centre for Nanoscale BioPhotonics, Griffith University, Gold Coast, Queensland, Australia.
⁸ Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, Centros, Singapore.
⁹ Beilstein-Institut, Trakehner Str. 7-9, Frankfurt am Main, Germany.

PMID: 30778580
DOI: 10.1093/glycob/cwz009

Abstract

The Minimum Information Required for a Glycomics Experiment (MIRAGE) is an initiative created by experts in the fields of glycobiology, glycoanalytics and glycoinformatics to design guidelines that improve the reporting and reproducibility of glycoanalytical methods. Previously, the MIRAGE Commission has published guidelines for describing sample preparation methods and the reporting of glycan array and mass spectrometry techniques and data collections. Here, we present the first version of guidelines that aim to improve the quality of the reporting of liquid chromatography (LC) glycan data in the scientific literature. These guidelines cover all aspects of instrument setup and modality of data handling and manipulation and is cross-linked with other MIRAGE recommendations. The most recent version of the MIRAGE-LC guidelines is freely available at the MIRAGE project website doi:10.3762/mirage.4.

Keywords: data sharing; glycomics; guidelines; liquid chromatography.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Liquid
Glycomics*
Humans
Polysaccharides / analysis*

Substances

Polysaccharides