Expanding the Spectrum of Light-Driven Peroxygenase Reactions

Sébastien J-P Willot; Elena Fernández-Fueyo; Florian Tieves; Milja Pesic; Miguel Alcalde; Isabel W C E Arends; Chan Beum Park; Frank Hollmann

doi:10.1021/acscatal.8b03752

Expanding the Spectrum of Light-Driven Peroxygenase Reactions

ACS Catal. 2019 Feb 1;9(2):890-894. doi: 10.1021/acscatal.8b03752. Epub 2018 Dec 18.

Authors

Sébastien J-P Willot¹, Elena Fernández-Fueyo¹, Florian Tieves¹, Milja Pesic¹, Miguel Alcalde², Isabel W C E Arends³, Chan Beum Park⁴, Frank Hollmann¹

Affiliations

¹ Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629 HZ Delft, The Netherlands.
² Department of Biocatalysis, Institute of Catalysis, CSIC, 28049 Madrid, Spain.
³ Faculty of Science, University of Utrecht, 3584 CD Utrecht, The Netherlands.
⁴ Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), 335 Science Road, Daejeon 305-701, Republic of Korea.

Abstract

Peroxygenases require a controlled supply of H₂O₂ to operate efficiently. Here, we propose a photocatalytic system for the reductive activation of ambient O₂ to produce H₂O₂ which uses the energy provided by visible light more efficiently based on the combination of wavelength-complementary photosensitizers. This approach was coupled to an enzymatic system to make formate available as a sacrificial electron donor. The scope and current limitations of this approach are reported and discussed.