Highly synergistic effects on ammonium removal by the co-system of Pseudomonas stutzeri XL-2 and modified walnut shell biochar

Yang Yu; Qiang An; Ying Zhou; Shuman Deng; Yue Miao; Bin Zhao; Li Yang

doi:10.1016/j.biortech.2019.02.037

Highly synergistic effects on ammonium removal by the co-system of Pseudomonas stutzeri XL-2 and modified walnut shell biochar

Bioresour Technol. 2019 May:280:239-246. doi: 10.1016/j.biortech.2019.02.037. Epub 2019 Feb 8.

Authors

Yang Yu¹, Qiang An², Ying Zhou¹, Shuman Deng¹, Yue Miao³, Bin Zhao⁴, Li Yang¹

Affiliations

¹ Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, PR China.
² Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, PR China; National Centre for International Research of Low-carbon and Green Buildings, Chongqing University, Chongqing 400045, PR China. Electronic address: anqiang@cqu.edu.cn.
³ Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, PR China. Electronic address: miaoyue2017@cqu.edu.cn.
⁴ Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, PR China; National Centre for International Research of Low-carbon and Green Buildings, Chongqing University, Chongqing 400045, PR China. Electronic address: binzhao11@cqu.edu.cn.

PMID: 30772636
DOI: 10.1016/j.biortech.2019.02.037

Abstract

Pseudomonas stutzeri strain XL-2 presented efficient ammonium removal due to heterotrophic nitrification-aerobic denitrification. The modified walnut shell biochar also showed ammonium adsorption due to chemical interaction. The complex of modified biochar and strain XL-2 exhibited excellent synergistic effects on ammonium removal, especially in unfavorable environment. The maximum average ammonium removal rate of the complex was 4.40 mg·L^-1·h^-1, which was 3.01 times higher than that of pure bacteria and 5.57 times higher than that of biochar. A large number of irregular pores and hydrophilic functional groups promoted the immobilization of strain XL-2 on biochar. Adsorption of ammonium, high specific surface area and release of Mg²⁺ by biochar enhanced biodegradation of strain XL-2. Approximately 96.34%-98.73% of ammonium was removed in a sequencing batch reactor (SBR) inoculating with the complex of strain XL-2 and biochar, which was much higher than the treatment efficiency of free bacteria in SBR.

Keywords: Adsorption; Ammonium removal; Bacteria; Biochar; Heterotrophic nitrification and aerobic denitrification.

MeSH terms

Aerobiosis
Ammonium Compounds / metabolism*
Charcoal / chemistry*
Denitrification
Heterotrophic Processes
Juglans / chemistry*
Nitrification
Nitrogen / metabolism
Pseudomonas stutzeri / metabolism*

Substances

Ammonium Compounds
biochar
Charcoal
Nitrogen