Detection of tyramine and tyrosinase activity using red region emission NaGdF4:Yb,Er@NaYF4 upconversion nanoparticles

Huihui Wang; Yunyun Lu; Lun Wang; Hongqi Chen

doi:10.1016/j.talanta.2019.01.079

Detection of tyramine and tyrosinase activity using red region emission NaGdF₄:Yb,Er@NaYF₄ upconversion nanoparticles

Talanta. 2019 May 15:197:558-566. doi: 10.1016/j.talanta.2019.01.079. Epub 2019 Jan 25.

Authors

Huihui Wang¹, Yunyun Lu¹, Lun Wang², Hongqi Chen³

Affiliations

¹ Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
² Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China. Electronic address: wanglun@mail.ahnu.edu.cn.
³ Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China. Electronic address: hq80chen@mail.ahnu.edu.cn.

PMID: 30771976
DOI: 10.1016/j.talanta.2019.01.079

Abstract

A selective, simple and environment-friendly type fluorescence sensing platform between upconversion nanoparticles (UCNPs) and melanin-like polymer utilizing the photoinduced electron-transfer (PET) mechanism was developed. The effective fluorescence quenching of UCNPs by melanin-like polymers could detect tyramine and tyrosinase (TYR) activity through the bio-catalysed oxidation of tyramine to the melanin-like polymer products. At optimal conditions, the range of linearity in detection was 0.167-33.3 μΜ for tyramine, and the limit of detection of tyramine was 0.026 μΜ. The linear range of TYR was 0.0033-1 U·mL^-1, and the limit of detection of TYR was 0.003 U·mL^-1. Furthermore, the strategy could be applied to real sample analysis with satisfactory results.

Keywords: Detection; NaGdF(4):Yb,Er@NaYF(4) UCNPs; Tyramine; Tyrosinase.

MeSH terms

Erbium / chemistry
Fluorescent Dyes / chemistry*
Fluorides / chemistry
Gadolinium / chemistry
Monophenol Monooxygenase / analysis*
Monophenol Monooxygenase / metabolism
Particle Size
Sodium / chemistry
Tyramine / analysis*
Ytterbium / chemistry
Yttrium / chemistry

Substances

Fluorescent Dyes
sodium yttriumtetrafluoride
Yttrium
Erbium
Sodium
Gadolinium
Monophenol Monooxygenase
Ytterbium
Fluorides
Tyramine