On-chip solid phase extraction and in situ optical detection

Long Li; Jibran Iqbal; Hui Li; Yuan Zhang; Lin Sun; Ying Zhu; Wanchao Chen; Yongqing Xi; Ting Wu; Yiping Du

doi:10.1016/j.talanta.2019.01.044

On-chip solid phase extraction and in situ optical detection

Talanta. 2019 May 15:197:299-303. doi: 10.1016/j.talanta.2019.01.044. Epub 2019 Jan 11.

Authors

Long Li¹, Jibran Iqbal², Hui Li³, Yuan Zhang¹, Lin Sun¹, Ying Zhu¹, Wanchao Chen¹, Yongqing Xi¹, Ting Wu¹, Yiping Du⁴

Affiliations

¹ Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
² College of Natural and Health Sciences, Zayed University, Abu Dhabi, UAE.
³ Department of Science and Engineering, Dehong Teachers' College, Xianchi Road 14, Mangshi 678400, China.
⁴ Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. Electronic address: yipingdu@ecust.edu.cn.

PMID: 30771939
DOI: 10.1016/j.talanta.2019.01.044

Abstract

A device was designed integrating solid phase extraction (SPE) and in situ optical detection techniques. A chip assembled in the device to carry out SPE was enclosed in a fully reflective aluminum box to avoid loss of radiation flux in the optical measurement. The spectrum was measured on the SPE material directly after SPE. The device offers simple analytical operation without any elution step comparing with the traditional analytical methods with complicated procedures. The device and the developed analytical technique was used for the determination of rhodamine B and benzo[a]pyrene with UV-visible diffuse reflectance and solid phase fluorescence spectroscopies, respectively. Under optimal conditions, standard curves of rhodamine B and benzo[a]pyrene showed a linear relationship in concentration ranges of 10-800 μg L^-1 (R² = 0.99) and 0.07-3.33 μg L^-1 (R² = 0.99), respectively. The limit of detections (LOD) were 7 μg L^-1 and 0.02 μg L^-1. The proposed device was successfully employed to quantify rhodamine B and benzo[a]pyrene in tap water samples. The results showed that the recoveries were 90-105% and the relative standard deviations (RSDs) were 2.53-5.55%, indicating that the device and analytical method is capable of trace analytes for rapid quantitative analysis in various samples.

Keywords: Fluorescence spectroscopy; In situ; On-chip SPE; Optical detection; UV–Vis spectroscopy.