Black phosphorus nanosheets based sensitive protease detection and inhibitor screening

Zhenzhen Hu; Yongxin Li; Ejaz Hussain; Xinan Huang; Yunyi Zhang; Niu Niu; Sohail Anjum Shahzad; Cong Yu

doi:10.1016/j.talanta.2019.01.023

Black phosphorus nanosheets based sensitive protease detection and inhibitor screening

Talanta. 2019 May 15:197:270-276. doi: 10.1016/j.talanta.2019.01.023. Epub 2019 Jan 8.

Authors

Zhenzhen Hu¹, Yongxin Li², Ejaz Hussain¹, Xinan Huang³, Yunyi Zhang⁴, Niu Niu², Sohail Anjum Shahzad⁵, Cong Yu⁶

Affiliations

¹ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
² State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of Science and Technology of China, Hefei 230026, PR China.
³ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
⁴ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; School of Materials Science and Engineering, Tianjin University, Tianjin 300072, PR China.
⁵ Department of Chemistry, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, Pakistan.
⁶ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China; University of Science and Technology of China, Hefei 230026, PR China. Electronic address: congyu@ciac.ac.cn.

PMID: 30771934
DOI: 10.1016/j.talanta.2019.01.023

Abstract

Proteases, as one of the most significant kind of digestive enzymes, are closely related to a variety of physiological processes and diseases. Herein, a black phosphorus nanosheets (BPs) based sensitive fluorometric method for protease detection and inhibitor screening is proposed. The aqueous solution of perylene probe (probe 1) displays a strong fluorescence. BPs, as novel discovered two-dimensional (2D) materials, can adsorb probe 1 through electrostatic interactions, which causes fluorescence quenching of probe 1. Histone can control the interactions between the perylene probe and BPs, which can be further regulated by the introduction of a protease. Thus, the protease activity can be monitored by detecting the fluorescence intensity changes of probe 1. The method is label free, sensitive and selective. As low as 1 ng/mL trypsin can be easily detected.

Keywords: Black phosphorus nanosheets; Fluorescence; Inhibitor; Perylene probe; Protease; Trypsin.

MeSH terms

Fluorescence
Fluorescent Dyes / chemistry*
Fluorescent Dyes / pharmacology
Humans
Nanostructures / chemistry*
Peptide Hydrolases / analysis*
Peptide Hydrolases / metabolism
Perylene / chemistry*
Perylene / pharmacology
Phosphorus / chemistry*

Substances

Fluorescent Dyes
Phosphorus
Perylene
Peptide Hydrolases