Ultrasound-enhanced scintillation proximity assay for rapid diagnostics

So-Young Lee; Jae-Cheong Lim; Eun-Ha Cho; Seung-Kon Lee; Sung-Hee Jung

doi:10.1016/j.ab.2019.02.011

Ultrasound-enhanced scintillation proximity assay for rapid diagnostics

Anal Biochem. 2019 Apr 1:570:51-55. doi: 10.1016/j.ab.2019.02.011. Epub 2019 Feb 13.

Authors

So-Young Lee¹, Jae-Cheong Lim², Eun-Ha Cho², Seung-Kon Lee², Sung-Hee Jung²

Affiliations

¹ Radioisotope Research Division, Korea Atomic Energy Research Institute (KAERI), Daejeon, 305-353, Republic of Korea. Electronic address: solee31@kaeri.re.kr.
² Radioisotope Research Division, Korea Atomic Energy Research Institute (KAERI), Daejeon, 305-353, Republic of Korea.

PMID: 30771337
DOI: 10.1016/j.ab.2019.02.011

Abstract

Scintillation proximity assay (SPA) is a type of radioimmunoassay (RIA). We apply ultrasound enhancement to the general SPA. All assay procedures, including the antibody coating and radiolabeled antigen binding are achieved by simply mixing then standing for 5 min in an ultrasound chamber. No additional incubation time is required. To further demonstrate the capability of the UE-SPA, a quantitative measurement of CD55 in various grades of colon tumors was assessed on human tissue slides. The results showed a significant correlation between CD55 expression and tumorigenesis. In conclusion, we confirmed that UE-SPA is a reliable, rapid and alternative to RIA.

Keywords: CD55; Immunoassay; Iodine-125; Scintillation proximity assay; Ultrasound.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal / immunology
CD55 Antigens / analysis*
CD55 Antigens / immunology
Colonic Neoplasms / metabolism
Colonic Neoplasms / pathology
Humans
Radioimmunoassay / methods*
Sonication

Substances

Antibodies, Monoclonal
CD55 Antigens