Generation and Analysis of Viral Vector-Mediated Rodent Models for Parkinson's Disease

Akua A Karikari; James B Koprich; Chi Wang Ip

doi:10.1007/978-1-4939-9124-2_21

Generation and Analysis of Viral Vector-Mediated Rodent Models for Parkinson's Disease

Methods Mol Biol. 2019:1948:271-286. doi: 10.1007/978-1-4939-9124-2_21.

Authors

Akua A Karikari¹, James B Koprich², Chi Wang Ip¹

Affiliations

¹ Department of Neurology, University Hospital of Würzburg, Würzburg, Germany.
² University Health Network, Krembil Research Institute, Toronto Western Hospital, Toronto, ON, Canada. jkoprich@uhnresearch.ca.

PMID: 30771185
DOI: 10.1007/978-1-4939-9124-2_21

Abstract

The use of viral vector-mediated α-synuclein-overexpressing rodent models for Parkinson's disease has become increasingly accepted to study associated pathology and to use as a platform for therapeutic efficacy testing. We here describe methods to generate such models and how to analyze them by behavioral assessments, histological investigations, and examination of nigrostriatal dopaminergic function.

Keywords: Dopamine; Mouse model; Neurodegeneration; Parkinson’s disease; Rat model; Substantia nigra.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Behavior, Animal
Biomarkers
Cell Count
Chromatography, High Pressure Liquid
Disease Models, Animal*
Dopamine / metabolism
Dopaminergic Neurons / metabolism
Dopaminergic Neurons / pathology
Genetic Vectors / genetics*
Immunohistochemistry
Parkinson Disease / etiology*
Parkinson Disease / metabolism*
Parkinson Disease / pathology
Rats
Rodentia
Stereotaxic Techniques
Substantia Nigra / metabolism
Substantia Nigra / pathology
Viruses / genetics*

Substances

Biomarkers
Dopamine