Increased Phenolic Content and Enhanced Antioxidant Activity in Fermented Glutinous Rice Supplemented with Fu Brick Tea

Molecules. 2019 Feb 14;24(4):671. doi: 10.3390/molecules24040671.

Abstract

Glutinous rice-based foods have a long history are consumed worldwide. They are also in great demand for the pursuit of novel sensory and natural health benefits. In this study, we developed a novel fermented glutinous rice product with the supplementation of Fu brick tea. Using in vitro antioxidant evaluation and phenolic compounds analysis, fermentation with Fu brick tea increased the total phenolic content and enhanced the antioxidant activity of glutinous rice, including scavenging of 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radical, 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical, and hydroxyl radical, ferric-reducing antioxidant power, and ferric ion reducing power and iron chelating capability. Besides, compared with traditional fermented glutinous rice, this novel functional food exhibited a stronger activity for protecting DNA against hydroxyl radical-induced oxidation damage. Quantitative analysis by HPLC identified 14 compounds covering catechins and phenolic acids, which were considered to be positively related to the enhanced antioxidant capability. Furthermore, we found that 80% ethanol was a suitable extract solvent compared with water, because of its higher extraction efficiency and stronger functional activities. Our results suggested that this novel fermented glutinous rice could serve as a nutraceutical food/ingredient with special sensory and functional activities.

Keywords: DNA damage protection; Fu brick tea; antioxidant activities; fermented glutinous rice; phenolic compounds; sensory evaluation.

MeSH terms

  • Antioxidants / chemistry*
  • Benzothiazoles / chemistry
  • Biphenyl Compounds / chemistry
  • Catechols / chemistry
  • China
  • Chromatography, High Pressure Liquid
  • DNA Damage / drug effects
  • Fermentation
  • Free Radical Scavengers / chemistry
  • Humans
  • Hydroxyl Radical / chemistry
  • Iron Chelating Agents / chemistry*
  • Oryza / chemistry*
  • Oxidation-Reduction
  • Phenols / chemistry*
  • Picrates / chemistry
  • Sulfonic Acids / chemistry
  • Triterpenes / chemistry

Substances

  • 3alpha,19alpha,20beta,24,30-pentahydroxyurs-12-en-28-oic acid
  • Antioxidants
  • Benzothiazoles
  • Biphenyl Compounds
  • Catechols
  • Free Radical Scavengers
  • Iron Chelating Agents
  • Phenols
  • Picrates
  • Sulfonic Acids
  • Triterpenes
  • 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  • Hydroxyl Radical
  • 1,1-diphenyl-2-picrylhydrazyl
  • catechol