Wheat Grain Yield (GY) and quality are particularly susceptible to nitrogen (N) fertilizer management. However, in rain-fed Mediterranean environments, crop N requirements might be variable due to the effects of water availability on crop growth. Therefore, in-season crop N status assessment is needed in order to apply N fertilizer in a cost-effective way while reducing environmental impacts. Remote sensing techniques might be useful at assessing in-season crop N status. In this study, we evaluated the capacity of vegetation indices formulated using blue (B), green (G), red (R) and near-infrared (NIR) bands obtained with a consumer-grade camera to assess wheat N status. Chlorophyll Content Index (CCI) and fractional intercepted PAR (fIPAR) were measured at three phenological stages and GY and biomass were determined at harvest. Indices formulated using RG bands and the normalized difference vegetation index (NDVI) were significantly correlated with both CCI and fIPAR at the different phenological stage (0.71 < r < 0.81, P < 0.01). Moreover, indices formulated using RG bands were capable at differentiating unfertilized and fertilized plots. In addition, RGB indices and NDVI were found to be related to both crop biomass and GY at harvest, particularly when data were obtained at initial grain filling stage (r > 0.80, P < 0.01). Finally, RGB indices and NDVI obtained with a consumer-grade camera showed comparable capacity at assessing chlorophyll content and predicting both crop biomass and GY at harvest than those obtained with a spectroradiometer. This study highlights the potential of standard and modified consumer-grade cameras at assessing canopy traits related to crop N status and GY in wheat under Mediterranean conditions.