A proteomic profiling dataset of recombinant Chinese hamster ovary cells showing enhanced cellular growth following miR-378 depletion

Orla Coleman; Alan Costello; Michael Henry; Nga T Lao; Niall Barron; Martin Clynes; Paula Meleady

doi:10.1016/j.dib.2018.11.115

A proteomic profiling dataset of recombinant Chinese hamster ovary cells showing enhanced cellular growth following miR-378 depletion

Data Brief. 2018 Nov 27:21:2679-2688. doi: 10.1016/j.dib.2018.11.115. eCollection 2018 Dec.

Authors

Orla Coleman¹, Alan Costello¹, Michael Henry¹, Nga T Lao¹, Niall Barron^{2

3}, Martin Clynes¹, Paula Meleady¹

Affiliations

¹ National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Ireland.
² National Institute for Bioprocess Research and Training, Blackrock Co., Dublin, Ireland.
³ University College Dublin, Belfield, Dublin 4, Ireland.

Abstract

The proteomic data presented in this article provide supporting information to the related research article "Depletion of endogenous miRNA-378-3p increases peak cell density of CHO DP12 cells and is correlated with elevated levels of Ubiquitin Carboxyl-Terminal Hydrolase 14" (Costello et al., in press) [1]. Control and microRNA-378 depleted CHO DP12 cells were profiled using label-free quantitative proteomic profiling. CHO DP12 cells were collected on day 4 and 8 of batch culture, subcellular proteomic enrichment was performed, and subsequent fractions were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). Here we provide the complete proteomic dataset of proteins significantly differentially expressed by greater than 1.25-fold change in abundance between control and miR-378 depleted CHO DP12 cells, and the lists of all identified proteins for each condition.