Evidence of a Large Diversity of N-acyl-Homoserine Lactones in Symbiotic Vibrio fischeri Strains Associated with the Squid Euprymna scolopes

Microbes Environ. 2019 Mar 30;34(1):99-103. doi: 10.1264/jsme2.ME18145. Epub 2019 Feb 13.

Abstract

Vibrio fischeri possesses a complex AHL-mediated Quorum-sensing (QS) system including two pathways, LuxI/R (3-oxo-C6-HSL and C6-HSL) and AinS/R (C8-HSL), which are important for the regulation of physiological traits. Diverse QS-dependent functional phenotypes have been described in V. fischeri; however, AHL diversity is still underestimated. In the present study, we investigated AHL diversity in five symbiotic V. fischeri strains with distinct phenotypic properties using UHPLC-HRMS/MS. The results obtained (1) revealed an unexpectedly high diversity of signaling molecules, (2) emphasized the complexity of QS in V. fischeri, and (3) highlight the importance of understanding the specificity of AHL-mediated QS.

Keywords: Euprymna scolopes; N-acyl-homoserine lactone; Quorum sensing; Vibrio fischeri.

MeSH terms

  • Acyl-Butyrolactones / chemistry*
  • Acyl-Butyrolactones / metabolism
  • Aliivibrio fischeri / chemistry*
  • Aliivibrio fischeri / genetics
  • Animals
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Decapodiformes / microbiology*
  • Gene Expression Regulation, Bacterial
  • Genetic Variation
  • Phenotype
  • Quorum Sensing*
  • Species Specificity
  • Symbiosis

Substances

  • Acyl-Butyrolactones
  • Bacterial Proteins