Cryopreservation and plant regeneration from somatic embryos of Pinus patula

C S Ford; N B Jones; J van Staden

doi:10.1007/s002990050781

Cryopreservation and plant regeneration from somatic embryos of Pinus patula

Plant Cell Rep. 2000 May;19(6):610-615. doi: 10.1007/s002990050781.

Authors

C S Ford¹, N B Jones¹, J van Staden¹

Affiliation

¹ NU Research Unit for Plant Growth and Development, Department of Botany, University of Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa e-mail: vanstadenj@botany.unp.ac.za Fax: +27-331-2605897, , , , , , ZA.

PMID: 30754825
DOI: 10.1007/s002990050781

Abstract

Embryogenic tissue of Pinus patula Scheide et Deppe was cryopreserved for 8 weeks using sorbitol and dimethylsulfoxide (DMSO) as cryoprotectants. Results indicate that 0.3 M sorbitol and 5% DMSO had the best cryoprotecting effect. The recovered tissue initially underwent a lag phase but then continued to proliferate normally on MSG3 maintenance medium. Recovered tissue was placed onto MSG5 maturation medium, and embryos were isolated and germinated. Plantlet regeneration from the recovered tissue was achieved.

Keywords: Embryogenic tissue; Key words Cryopreservation; Pinus patula.