Soybean rust (SBR), caused by Phakopsora pachyrhizi, has the potential to be an economic threat to U.S. soybean production after its arrival to the continental United States in 2004. The use of fungicides to control SBR may be problematic due to the large acreage that needs to be protected, the high costs of fungicides, and the cost of application. Cultural practices such as the use of reduced seed rates, increased row widths, and row orientation to the sun have been prescribed as environmental modifications that create a microclimate less conducive to foliar disease development. Therefore, our objective was to determine the influence of different periods of leaf wetness and respective microenvironments on infection and rust development on soybean plants in the field. A misting irrigation system was developed and applied on MGV soybean for 1 min every 30 min for 0-, 6-, 12-, and 18-h periods. This study indicates that extended periods of leaf wetness (18 h) increase disease severity and the rate of spread of the disease in the upper canopy. These results, in combination with spore monitoring, may be used to refine models of pathogen reproduction, prediction, and risk in a certain regions.