Genome Mining of Streptomyces atratus SCSIO ZH16: Discovery of Atratumycin and Identification of Its Biosynthetic Gene Cluster

Changli Sun; Zhijie Yang; Chunyan Zhang; Zhiyong Liu; Jianqiao He; Qing Liu; Tianyu Zhang; Jianhua Ju; Junying Ma

doi:10.1021/acs.orglett.9b00208

Genome Mining of Streptomyces atratus SCSIO ZH16: Discovery of Atratumycin and Identification of Its Biosynthetic Gene Cluster

Org Lett. 2019 Mar 1;21(5):1453-1457. doi: 10.1021/acs.orglett.9b00208. Epub 2019 Feb 12.

Authors

Changli Sun¹, Zhijie Yang^{1

2}, Chunyan Zhang^{1

2}, Zhiyong Liu³, Jianqiao He^{1

2}, Qing Liu^{1

4}, Tianyu Zhang^{3

2}, Jianhua Ju^{1

2}, Junying Ma¹

Affiliations

¹ CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology , South China Sea Institute of Oceanology, Chinese Academy of Sciences , Guangzhou 510301 , China.
² University of Chinese Academy of Sciences , 19 Yuquan Road , Beijing 100049 , China.
³ Tuberculosis Research Laboratory, State Key Laboratory of Respiratory Disease , Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences , Guangzhou 510530 , China.
⁴ State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences , Sun Yat-Sen University , Guangzhou 510275 , China.

PMID: 30746943
DOI: 10.1021/acs.orglett.9b00208

Abstract

Genome mining of the deep sea-derived Streptomyces atratus SCSIO ZH16 enabled the activation of a cyclodepsipeptide gene cluster and isolation of its cinnamic acid-bearing product, atratumycin (1). Atratumycin's structure was elucidated on the basis of extensive spectroscopic experiments, X-ray data, and Marfey's method; a plausible biosynthesis and tailoring modification of 1 are also proposed and investigated. Additionally, atratumycin is active against Mycobacteria tuberculosis H37Ra and H37Rv with MICs of 3.8 and 14.6 μM, respectively.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Microbial Sensitivity Tests
Molecular Structure
Multigene Family
Mycobacterium tuberculosis / chemistry*
Mycobacterium tuberculosis / drug effects
Peptides, Cyclic / chemistry
Peptides, Cyclic / pharmacology*
Streptomyces / chemistry
Streptomyces / genetics*

Substances

Peptides, Cyclic