Preparation of photosensitive diazotized poly (vinyl alcohol-b-styrene) covalent capillary coatings for capillary electrophoresis separation of proteins

Abstract

In this paper, we have developed a novel method for the preparation of covalently connected capillary coatings in which diazotized poly (vinyl alcohol-b-styrene) (diazo-P(VA-b-St)) was used as a photosensitive coating agent. Firstly, the diazo-P(VA-b-St) coating was self-assembled on the inner surface of the capillary, and then irradiated by ultraviolet (UV) light to convert the ionic bonding into covalent bonding through the unique photochemical reaction of diazo groups. The covalently connected coatings inhibited the protein adsorption on the inner surface of the capillary, as a result, the baseline protein separation of ribonuclease A (RNase A), lysozyme (Lyz) and bovine serum albumin (BSA) were attained by utilizing the capillary electrophoresis (CE). The covalently connected diazo-P(VA-b-St) capillary coatings have greater CE separation performance with magnificent repeatability and enhanced stability, when compared with non-covalently coated or bare capillaries. This strategy to synthesize photosensitive diazo-P(VA-b-St) capillary coatings for their use in capillary electrophoresis separation of proteins is highly environment-friendly as it does not involve the use of extremely noxious and moisture penetrating coatings of silane.

Keywords: Capillary coating; Capillary electrophoresis; Diazotized polymer; Photosensitive; Protein; Separation.