Multiplexing protein and gene level measurements on a single Luminex platform

Methods. 2019 Apr 1:158:27-32. doi: 10.1016/j.ymeth.2019.01.018. Epub 2019 Feb 8.

Abstract

Background: The ability to simultaneously measure multiple secreted proteins and the corresponding gene expression levels from a single sample is valuable for comprehensive analysis. Bottlenecks to traditional immunoassays and gene expression assays include large sample consumption, time consuming experimental procedures, and complex data analysis.

Method and results: Here, we demonstrate two high-throughput assays measuring both messenger RNA (mRNA) expression and proteins in a single sample run on a Luminex platform. Human peripheral blood mononuclear cells (hPBMCs) were treated with lipopolysaccharide (LPS) and harvested at 24 and 72 h. Samples were assayed with the ProcartaPlex Human Immune Monitoring 65-plex Panel for protein and corresponding mRNA targets on a QuantiGene Human 80-plex Panel.

Conclusion: Multiplexing ProcartaPlex and QuantiGene Plex assays provide a broad survey of protein levels and gene expression networks.

Keywords: Biomarker discovery; Luminex; ProcartaPlex; QuantiGene; Screening; mRNA expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cytokines / analysis
  • Cytokines / metabolism
  • Gene Expression Profiling / instrumentation
  • Gene Expression Profiling / methods*
  • High-Throughput Screening Assays / instrumentation
  • High-Throughput Screening Assays / methods*
  • Humans
  • Intercellular Signaling Peptides and Proteins / analysis
  • Intercellular Signaling Peptides and Proteins / metabolism
  • Leukocytes, Mononuclear
  • Microspheres
  • Proteomics / instrumentation
  • Proteomics / methods*
  • RNA, Messenger / analysis
  • RNA, Messenger / metabolism

Substances

  • Cytokines
  • Intercellular Signaling Peptides and Proteins
  • RNA, Messenger