Time-Lapse Technologies and 4D Imaging of Kidney Development

Methods Mol Biol. 2019:1926:163-167. doi: 10.1007/978-1-4939-9021-4_14.

Abstract

Time-lapse imaging is a technique of frequent imaging and following a course of a process. Because the development of the embryonic kidney can proceed ex vivo after dissection, it is possible to study the morphogenesis by culturing the kidney in the onstage incubator of a microscope and follow the developmental process by imaging. Confocal microscopes and other three-dimensional imaging systems offer the possibility for tracking the development process in four dimensions-3D and the time.

Keywords: Kidney development; Time-lapse imaging; Tissue culture.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Humans
  • Kidney / cytology*
  • Microscopy, Confocal
  • Time-Lapse Imaging / methods*
  • Tissue Culture Techniques / methods