Adjuvant and antigenic properties of Mycobacterium avium subsp. paratuberculosis on experimental autoimmune encephalomyelitis

Davide Cossu; Kazumasa Yokoyama; Tamami Sakanishi; Eiichi Momotani; Nobutaka Hattori

doi:10.1016/j.jneuroim.2019.01.013

Adjuvant and antigenic properties of Mycobacterium avium subsp. paratuberculosis on experimental autoimmune encephalomyelitis

J Neuroimmunol. 2019 May 15:330:174-177. doi: 10.1016/j.jneuroim.2019.01.013. Epub 2019 Jan 23.

Authors

Davide Cossu¹, Kazumasa Yokoyama², Tamami Sakanishi³, Eiichi Momotani⁴, Nobutaka Hattori⁵

Affiliations

¹ Juntendo University, Department of Neurology, Tokyo, Japan; Juntendo University, Advanced Research Institute for Health Science, Tokyo, Japan.
² Juntendo University, Department of Neurology, Tokyo, Japan; Juntendo University, Department of Treatment and Research in Multiple Sclerosis and Neuro-intractable disease, Tokyo, Japan. Electronic address: kazumasa@juntendo.ac.jp.
³ Juntendo University, Division of Cell Biology, Tokyo, Japan.
⁴ Comparative Medical Research Institute, Laboratory of Immunopathology, Tsukuba, Japan.
⁵ Juntendo University, Department of Neurology, Tokyo, Japan; Juntendo University, Department of Treatment and Research in Multiple Sclerosis and Neuro-intractable disease, Tokyo, Japan.

PMID: 30738572
DOI: 10.1016/j.jneuroim.2019.01.013

Abstract

Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of Johne's disease in ruminants, has been linked as a possible risk factor for human multiple sclerosis. In the current study we investigated the adjuvant effect of MAP on experimental autoimmune encephalomyelitis (EAE). Groups of C57BL/6 mice were actively immunized with myelin oligodendrocyte glycoprotein (MOG) _35-55 peptide emulsified in incomplete Freund's adjuvant modified containing heat-killed MAP (MIFA). MOG-MIFA immunized mice showed an early disease onset and more severe clinical scores in comparison with MOG-CFA immunized mice, demonstrating for the first time the adjuvant effect of MAP on EAE development.

Keywords: Adjuvant; C57BL/6J mice; Experimental autoimmune encephalomyelitis; Mycobacterium avium subsp. paratuberculosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Immunologic / administration & dosage*
Animals
Encephalomyelitis, Autoimmune, Experimental / immunology*
Encephalomyelitis, Autoimmune, Experimental / pathology
Encephalomyelitis, Autoimmune, Experimental / therapy*
Female
Mice
Mice, Inbred C57BL
Mycobacterium avium subsp. paratuberculosis / immunology*

Substances

Adjuvants, Immunologic