Phentotypic, gentotypic antimicrobial resistance and pathogenicity of Salmonella enterica serovars Typimurium and Enteriditis in poultry and poultry products

Sher Bahadar Khan; Mumtaz Ali Khan; Irshad Ahmad; Tayyab Ur Rehman; Shahid Ullah; Rahim Dad; Asad Sultan; Atta Muhammad Memon

doi:10.1016/j.micpath.2019.01.046

Phentotypic, gentotypic antimicrobial resistance and pathogenicity of Salmonella enterica serovars Typimurium and Enteriditis in poultry and poultry products

Microb Pathog. 2019 Apr:129:118-124. doi: 10.1016/j.micpath.2019.01.046. Epub 2019 Feb 7.

Authors

Sher Bahadar Khan¹, Mumtaz Ali Khan², Irshad Ahmad³, Tayyab Ur Rehman³, Shahid Ullah⁴, Rahim Dad⁵, Asad Sultan⁶, Atta Muhammad Memon⁷

Affiliations

¹ Department of Animal Health, The University of Agriculture, Peshawar, Pakistan. Electronic address: drsher@aup.edu.pk.
² Civil Veterinary Hospital, SherGarh, Livestock and Dairy Development, Khyber Pakhtunkhwa, Pakistan; University of Veterinary and Animal Sciences, Lahore, Pakistan.
³ Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan.
⁴ College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, 518060, Guangdong, China.
⁵ Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, Pakistan.
⁶ Department of Poultry Sciences, The University of Agriculture, Peshawar, Pakistan.
⁷ Livestock and Fisheries Department, Government of Sindh, Pakistan.

PMID: 30738177
DOI: 10.1016/j.micpath.2019.01.046

Abstract

For detection and isolation of Salmonella enterica, 650 meat and tissue samples were processed using Rappaport-Vassiliadis Enrichment broth and Salmonella Chromogenic agar followed by confirmation through specific antisera and polymerase chain reaction (PCR) targeting their Specific Serovar Genomic Regions (SSGRS). Isolates were tested for 15 antibiotics (CRO, AMX, GEN, STR, TET, CHL, CLR, LVX, OFX, GAT, CIP, SXT, AMP, LIN and AZM) according to the disc diffusion method and antimicrobial resistant genes (tet(A), tet(B), tet(C), strA/strB, aadA, aac(3)IV), aadB, sul1, sul2 and sul3, blaCMY-2, blaTEM and blaSHV) using PCR. The overall prevalence of Salmonella enterica was 12%, being higher in markets (15%) as compared to poultry farms (37.2%). The MPN of all positive meat and tissue samples was found 3.6 MPN/gram (0.17-18). A total of 234 isolates were obtained, serovar Typimurium (139) and Enteridits (95) were the most prevalent. Antimicrobial resistance patterns were different in different serovars according to origin of Salmonella isolates. The overall isolates were highly resistant for LIN (93.1%, 218/234) followed by AMX (80%, 187/234), AMP (74.3%, 174/234), TET (64.5%, 151/234) and STR (64.5%, 151/234). Overall, the most common ARG was bla_TEM (76%, 178/234), followed by bla_SHV (71.7%, 168/234), tet(A) (64%, 151/234) and tet(B) (64%, 150/234), while the least ARG was aadB (7.2%, 17/234). Both Typimurium and Enteridits were tested in the Balb/C mice for pathogenicity. Both Typimurium and Enteridits were found to cause successful colonization, 100% morbidity but Enteriditis were found to cause 33% mortality.

Keywords: Antibiotic resistance; Antibiotic resistant genes; Pathogenecity; Poultry; Poultry products; Salmonella; Serovar.

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Bacteriological Techniques
Drug Resistance, Bacterial*
Female
Genes, Bacterial
Mice, Inbred BALB C
Models, Animal
Polymerase Chain Reaction
Poultry
Poultry Diseases / microbiology*
Poultry Products / microbiology*
Prevalence
Salmonella Infections, Animal / epidemiology
Salmonella Infections, Animal / microbiology*
Salmonella enteritidis / drug effects
Salmonella enteritidis / genetics
Salmonella enteritidis / isolation & purification*
Salmonella typhimurium / drug effects
Salmonella typhimurium / genetics
Salmonella typhimurium / isolation & purification*
Survival Analysis

Substances

Anti-Bacterial Agents