Germ Cell Transplantation in Avian Species

Young Hyun Park; Young Min Kim; Jae Yong Han

doi:10.1007/978-1-4939-9009-2_19

Germ Cell Transplantation in Avian Species

Methods Mol Biol. 2019:1920:317-326. doi: 10.1007/978-1-4939-9009-2_19.

Authors

Young Hyun Park¹, Young Min Kim¹, Jae Yong Han²

Affiliations

¹ Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea (Republic of).
² Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea (Republic of). jaehan@snu.ac.kr.

PMID: 30737700
DOI: 10.1007/978-1-4939-9009-2_19

Abstract

Germ cell transplantation technology has played a critical role in germline modification and preservation of genetic resources. Several germ cell transplantation systems have been developed, including sperm, oocyte, or germline stem cell transplantation systems in mammals. Meanwhile, in avian species, this has mostly relied on primordial germ cell (PGC) transplantation for efficient germline transmission. In this chapter, we describe how to isolate PGCs from avian embryos and produce germline chimeras through transplantation of donor PGCs to recipient embryos.

Keywords: Aves; Germline chimera; Primordial germ cells; Transplantation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Movement
Cell Separation / methods
Cell Transplantation* / methods
Chickens*
Crosses, Genetic
Embryo, Nonmammalian
Fluorescent Antibody Technique
Germ Cells / cytology*
Germ Cells / transplantation*
Immunohistochemistry
Immunomagnetic Separation / methods