Expression of an antisense 1-aminocyclopropane-1-carboxylate oxidase gene stimulates shoot regeneration in Cucumis melo

M B Amor; M Guis; A Latché; M Bouzayen; J-C Pech; J-P Roustan

doi:10.1007/s002990050447

Expression of an antisense 1-aminocyclopropane-1-carboxylate oxidase gene stimulates shoot regeneration in Cucumis melo

Plant Cell Rep. 1998 Apr;17(6-7):586-589. doi: 10.1007/s002990050447.

Authors

M B Amor¹, M Guis¹, A Latché¹, M Bouzayen¹, J-C Pech¹, J-P Roustan¹

Affiliation

¹ Ecole Nationale Supérieure Agronomique de Toulouse, UA INRA, 145 avenue de Muret, 31076 Toulouse cedex, France Fax no.: +33-5-62-13-65-41 E-mail: roustan@flora.ensat.fr, , , , , , FR.

PMID: 30736641
DOI: 10.1007/s002990050447

Abstract

The role of ethylene in shoot regeneration was investigated using transgenic Cucumis melo plants expressing an antisense 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene. ACC oxidase catalyses the last step of ethylene biosynthesis. Leaf and cotyledon explants from the transgenic plants exhibited low ACC oxidase activity and ethylene production, whereas the regeneration capacity of the tissues was greatly enhanced (3.5- and 2.8-fold, respectively) compared to untransformed control tissues. Addition of ethylene released by 50 or 100 µM 2-chloroethylphosphonic acid dramatically reduced the shoot regeneration rate of the transgenic tissues. The results clearly demonstrate that ethylene plays an important role in C. melo morphogenesis in vitro.

Keywords: 1-Aminocyclopropane-1-carboxylate oxidase; Antisense RNA; Ethylene; Key wordsCucumis melo; Transgenic plant.