Inhibitor structure-guided design and synthesis of near-infrared fluorescent probes for monoamine oxidase A (MAO-A) and its application in living cells and in vivo

Chem Commun (Camb). 2019 Feb 21;55(17):2477-2480. doi: 10.1039/c8cc10084e.

Abstract

A series of near-infrared fluorescent probes based on inhibitor (clorgyline) structure-guided design were synthesized for the specific detection of MAO-A in cells and in vivo. Moreover, we have successfully used the high specificity of one of the probes to visualize MAO-A activity in zebrafish and tumor tissue for the first time.

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Clorgyline / analogs & derivatives*
  • Clorgyline / chemical synthesis
  • Clorgyline / toxicity
  • Drug Design
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / toxicity
  • Humans
  • Limit of Detection
  • Mice, Inbred BALB C
  • Microscopy, Fluorescence / methods
  • Molecular Structure
  • Monoamine Oxidase / analysis*
  • Monoamine Oxidase / metabolism*
  • Monoamine Oxidase Inhibitors / chemical synthesis
  • Monoamine Oxidase Inhibitors / chemistry*
  • Monoamine Oxidase Inhibitors / toxicity
  • Zebrafish

Substances

  • Fluorescent Dyes
  • Monoamine Oxidase Inhibitors
  • Monoamine Oxidase
  • Clorgyline