Abstract
Transcription is a process by which the rate of RNA synthesis is regulated. Here we describe the techniques for carrying out promoter-reporter assays, electrophoretic mobility shift assays, chromosome conformation capture (3C) assays, chromatin immunoprecipitation assays, and CRISPR-Cas9 assay, five commonly used methods for studying and altering gene transcription.
Keywords:
CRISPR-Cas9; ChIP; Chromatin; Chromosome conformation capture (3C) technology; EMSA; Gene; Immunoprecipitation; Promoter; Transcription.
MeSH terms
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Bone and Bones / cytology*
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CRISPR-Cas Systems / genetics
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Cell Culture Techniques / instrumentation
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Cell Culture Techniques / methods
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Cell Line, Tumor
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Cell Separation / instrumentation
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Cell Separation / methods
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Chromatin Immunoprecipitation / instrumentation
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Chromatin Immunoprecipitation / methods*
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Electrophoretic Mobility Shift Assay / instrumentation
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Electrophoretic Mobility Shift Assay / methods*
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Flow Cytometry / instrumentation
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Flow Cytometry / methods
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Gene Editing / instrumentation
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Gene Editing / methods
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Gene Expression Regulation*
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Genes, Reporter / genetics
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Humans
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Promoter Regions, Genetic / genetics
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Transcription, Genetic*