2D and 3D In Vitro Co-Culture for Cancer and Bone Cell Interaction Studies

Silvia Marino; Ryan T Bishop; Daniëlle de Ridder; Jesus Delgado-Calle; Michaela R Reagan

doi:10.1007/978-1-4939-8997-3_5

2D and 3D In Vitro Co-Culture for Cancer and Bone Cell Interaction Studies

Methods Mol Biol. 2019:1914:71-98. doi: 10.1007/978-1-4939-8997-3_5.

Authors

Silvia Marino¹, Ryan T Bishop², Daniëlle de Ridder², Jesus Delgado-Calle³, Michaela R Reagan⁴

Affiliations

¹ Division Hematology Oncology, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA. simarino@iu.edu.
² Department of Oncology and Metabolism, Medical School, University of Sheffield, Sheffield, UK.
³ Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN, USA.
⁴ Center for Molecular Medicine, Maine Medical Centre Research Institute, Scarborough, ME, USA.

PMID: 30729461
DOI: 10.1007/978-1-4939-8997-3_5

Abstract

Co-culture assays are used to study the mutual interaction between cells in vitro. This chapter describes 2D and 3D co-culture systems used to study cell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most commonly used cell culture techniques, functional assays, and gene expression.

Keywords: Cancer cells; In vitro co-culture; Osteoblasts; Osteoclasts; Osteocytes.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Adipocytes / physiology
Animals
Bombyx
Bone Marrow Cells / physiology*
Cell Communication / physiology*
Cell Differentiation / physiology
Cell Line, Tumor
Coculture Techniques / instrumentation
Coculture Techniques / methods*
Humans
Mice
Osteoclasts / physiology
Osteocytes / physiology
Primary Cell Culture / instrumentation
Primary Cell Culture / methods
Stromal Cells / physiology
Tissue Engineering / methods
Tissue Scaffolds

Abstract

Publication types

MeSH terms

Grants and funding