Isolation and Generation of Osteoclasts

Nadia Rucci; Alberta Zallone; Anna Teti

doi:10.1007/978-1-4939-8997-3_1

Isolation and Generation of Osteoclasts

Methods Mol Biol. 2019:1914:3-19. doi: 10.1007/978-1-4939-8997-3_1.

Authors

Nadia Rucci¹, Alberta Zallone², Anna Teti³

Affiliations

¹ Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy.
² Department of Basic Medical Sciences, Neuroscience and Sense Organs, University of Bari, Bari, Italy.
³ Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy. annamaria.teti@univaq.it.

PMID: 30729457
DOI: 10.1007/978-1-4939-8997-3_1

Abstract

This chapter describes the isolation, culture, and staining of osteoclasts. The key advantages of this assay are that it allows direct measurement of osteoclast number, bone resorption, as well as yielding good quantities of osteoclasts at defined stages of formation for molecular analysis. An additional focus of this chapter will be the generation of osteoclasts from less conventional animal species and cell lines.

Keywords: Bone loss; Bone remodeling; Bone resorption; Osteoclast.

MeSH terms

Animals
Biological Assay / instrumentation
Biological Assay / methods*
Bone Marrow Cells
Bone Resorption / pathology
Bone and Bones / cytology
Cell Differentiation*
Cell Line
Humans
Leukocytes, Mononuclear
Osteoclasts / physiology*
Primary Cell Culture / instrumentation
Primary Cell Culture / methods*
RANK Ligand / metabolism
Recombinant Proteins / metabolism

Substances

RANK Ligand
Recombinant Proteins