Transient expression of GUS and the 2S albumin gene from Brazil nut in peanut (Arachis hypogaea L.) seed explants using particle bombardment

C Lacorte; F J L Aragão; E R Almeida; E L Rech; E Mansur

doi:10.1007/BF01275502

Transient expression of GUS and the 2S albumin gene from Brazil nut in peanut (Arachis hypogaea L.) seed explants using particle bombardment

Plant Cell Rep. 1997 Jun;16(9):619-623. doi: 10.1007/BF01275502.

Authors

C Lacorte¹, F J L Aragão¹, E R Almeida¹, E L Rech¹, E Mansur²

Affiliations

¹ Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia, EMBRAPA - S.A.I.N. Parque Rural, P.O. Box 2372, CEP 70849-970, Brasilia, DF, Brazil.
² Dept. Biologia Celular e Genética, Universidade do Estado do Rio de Janeiro, R. Sáo Francisco Xavier 524, Rio de Janeiro, RJ, Brazil.

PMID: 30727606
DOI: 10.1007/BF01275502

Abstract

The effect of parameters involved in the transformation efficiency of peanut (Arachis hypogaea L.) seed tissues by direct gene transfer using a helium inflow particle bombardment device was evaluated. Transient gene expression was affected by both particle and DNA amounts, and was positively correlated with gene copy number, as determined byβ-glucuronidase (GUS) activity assays. No influence of plasmid size on GUS gene expression was observed. Transcriptional control of GUS by either the CaMV 35S or the 2S promoter from Brazil nut 2S albumin gene varied with the developmental stage of the seed and was approximately tenfold greater under the influence of the 35S promoter than under the 2S promoter. The gene products of both the Brazil nut methionine-rich 2S albumin and GUS genes under the transcriptional control of the 35S promoter were detected by ELISA assays.

Keywords: 2S albumin; Arachis hypogaea; Bertholetia excelsa; Biolistic; GUS; Transient expression.