Methionine Gamma Lyase from Clostridium sporogenes Increases the Anticancer Efficacy of Doxorubicin on A549 Cancer Cells In Vitro and Human Cancer Xenografts

Methods Mol Biol. 2019:1866:243-261. doi: 10.1007/978-1-4939-8796-2_18.

Abstract

The anticancer efficacy of methionine γ-lyase (MGL) from Clostridium sporogenes (C. sporogenes) is described. MGL was active against cancer cells in vitro and in vivo. Doxorubicin (DOX) and MGL were more effective on A549 human lung-cancer growth inhibition than either agent alone in vitro and in vivo.

Keywords: Anticancer efficacy; Clostridium sporogenes; Doxorubicin; Human tumor xenografts; Lung cancer; Methioninase.

MeSH terms

  • A549 Cells
  • Animals
  • Antineoplastic Agents / isolation & purification*
  • Antineoplastic Agents / pharmacology
  • Antineoplastic Agents / therapeutic use
  • Carbon-Sulfur Lyases / isolation & purification
  • Carbon-Sulfur Lyases / metabolism*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Cisplatin / pharmacology
  • Cisplatin / therapeutic use
  • Clostridium / enzymology*
  • Doxorubicin / pharmacology
  • Doxorubicin / therapeutic use
  • Enzyme Assays
  • Humans
  • MCF-7 Cells
  • Mice
  • Treatment Outcome
  • Xenograft Model Antitumor Assays*

Substances

  • Antineoplastic Agents
  • Doxorubicin
  • Carbon-Sulfur Lyases
  • L-methionine gamma-lyase
  • Cisplatin