A QuEChERS based extraction procedure coupled to UPLC-MS/MS detection for mycotoxins analysis in beer

Jesús M González-Jartín; Amparo Alfonso; Inés Rodríguez; María J Sainz; Mercedes R Vieytes; Luis M Botana

doi:10.1016/j.foodchem.2018.09.162

A QuEChERS based extraction procedure coupled to UPLC-MS/MS detection for mycotoxins analysis in beer

Food Chem. 2019 Mar 1:275:703-710. doi: 10.1016/j.foodchem.2018.09.162. Epub 2018 Sep 27.

Authors

Jesús M González-Jartín¹, Amparo Alfonso², Inés Rodríguez³, María J Sainz⁴, Mercedes R Vieytes⁵, Luis M Botana⁶

Affiliations

¹ Departamento de Farmacología, Facultad de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, Spain. Electronic address: jesus.gonzalez@usc.es.
² Departamento de Farmacología, Facultad de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, Spain. Electronic address: amparo.alfonso@usc.es.
³ Departamento de Farmacología, Facultad de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, Spain; Laboratario CIFGA S.A., Plaza de Santo Domingo, no. 20, 5a planta, 27001 Lugo, Spain. Electronic address: ines.rodriguez.filgueiras@usc.es.
⁴ Departamento de Producción Vegetal y Proyectos de Ingeniería, Facultad de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, Spain. Electronic address: mj.sainz@usc.es.
⁵ Departamento de Fisiología, Facultad de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, Spain. Electronic address: mmercedes.rodriguez@usc.es.
⁶ Departamento de Farmacología, Facultad de Veterinaria, Universidade de Santiago de Compostela, 27002 Lugo, Spain. Electronic address: luis.botana@usc.es.

PMID: 30724252
DOI: 10.1016/j.foodchem.2018.09.162

Abstract

A new method based on a QuEChERS extraction followed by the ultra-high liquid chromatography tandem mass spectrometry (UPLC-MS/MS) detection has been developed for the analysis of mycotoxin in beer. The method allows the identification and quantification of 23 mycotoxins with different chemical characteristic including regulated, emerging and masked compounds. A sample treatment procedure involving a QuEChERS extraction and dispersive solid-phase clean-up steps was applied. This protocol involves a new approach based on a sample concentration before the extraction. The method was in-house validated in terms of limits of detection (LODs), limits of quantification (LOQs), linearity, repeatability and recoveries. For most compounds, recoveries ranged from 70% to 110% with LOQs (from 0.038 to 30.43 µg/L) lower than the maximum residue levels established in European regulations. In general, acceptable performance characteristics were obtained fulfilling the current legislation. Therefore, the proposed method is appropriate for routine analysis of beer.

Keywords: Beer; Mycotoxin; QuEChERS; UPLC-MS/MS.

MeSH terms

Beer / analysis*
Chromatography, High Pressure Liquid
Limit of Detection
Mycotoxins / analysis*
Mycotoxins / isolation & purification
Solid Phase Extraction
Tandem Mass Spectrometry*

Substances

Mycotoxins